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Image Search Results
Journal: Journal of Biochemistry
Article Title: Roles of CUB and LDL receptor class A domain repeats of a transmembrane serine protease matriptase in its zymogen activation
doi: 10.1093/jb/mvs118
Figure Lengend Snippet: Western blot analysis of the pro-CLS-matWT and pro-CLS-matS805A variants. Medium samples of COS-1 cells transfected with pSec-pro-CLS-matWT alone (CLS-WT), pSec-pro-CLS-matWT and pSec-HAI-1NIK1LK2 (CLS-WT + HAI-1), pSec-pro-CLS-matS805A alone (CLS-S805A) and pSec-pro-CLS-matS805A and pSec- HAI-1NIK1LK2 (CLS-S805A + HAI-1) were subjected to reducing SDS-PAGE. After western transfer, the blot was probed with Spr992 or S-protein–HRP. The position to which pro-CLS-matWT and pSec-pro-CLS-matS805A migrated is indicated as ‘Proforms’ to the right of the right panel. The position to which HAI-1NIK1LK2 migrated is indicated to the right of the right panel. The molecular masses of the marker proteins are indicated in kilodaltons to the left of the left panel.
Article Snippet: When a sample of media conditioned by
Techniques: Western Blot, Transfection, SDS Page, Marker
Journal: Journal of Biochemistry
Article Title: Roles of CUB and LDL receptor class A domain repeats of a transmembrane serine protease matriptase in its zymogen activation
doi: 10.1093/jb/mvs118
Figure Lengend Snippet: Western blot analysis of the pro-LS-matWT and pro-LS-matS805A variants. Medium samples of COS-1 cells transfected with pSec-pro-LS-matWT alone (LS-WT), pSec-pro-LS-matWT and pSec-HAI-1NIK1LK2 (LS-WT + HAI-1), pSec-pro-LS-matS805A alone (LS-S805A) and pSec-pro-LS-matS805A and pSec-HAI-1NIK1LK2 (LS-S805A + HAI-1) were subjected to reducing SDS-PAGE. After western transfer, the blot was probed with Spr992 or S-protein–HRP. The 28-kDa position to which the SPCD part of two-chain pro-LS-matWT migrated is indicated as ‘SPCD of two-chain pro-LS-matWT’ to the right of the left panel. The 57-kDa position at which pro-LS-matS805A migrated is indicated as ‘pro-LS-matS805A’ to the right of the left panel. The 57-kDa position to which pro-LS-matWT and pro-LS-matS805A migrated is indicated as ‘Proforms’ to the right of the right panel. The position to which HAI-1NIK1LK2 migrated is indicated to the right of the right panel. The molecular masses of the marker proteins are indicated in kilodaltons to the left of both panels.
Article Snippet: When a sample of media conditioned by
Techniques: Western Blot, Transfection, SDS Page, Marker
Journal: Journal of Biochemistry
Article Title: Roles of CUB and LDL receptor class A domain repeats of a transmembrane serine protease matriptase in its zymogen activation
doi: 10.1093/jb/mvs118
Figure Lengend Snippet: Western blot analysis of the pro-S-matWT and pro-S-matS805A variants. Medium samples of COS-1 cells transfected with pSec-pro-S-matWT alone (S-WT), pSec-pro-S-matWT and pSec-HAI-1NIK1LK2 (S-WT + HAI-1), pSec-pro-S-matS805A alone (S-S805A) and pSec-pro-S-matS805A and pSec-HAI-1NIK1LK2 (S-S805A + HAI-1) were subjected to reducing SDS-PAGE. After western transfer, the blot was probed with Spr992 or S-protein–HRP. The 28-kDa position to which the SPCD part of two-chain pro-S-matWT migrated is indicated as ‘SPCD of two-chain pro-S-matWT’ to the right of the left panel. The 40-, 36- and 31-kDa positions to which pro-S-matWT and pro-S-matS805A species migrated are indicated as ‘Proforms’ to the right of the left and right panels. The position to which HAI-1NIK1LK2 migrated is indicated to the right of the right panel. The molecular masses of the marker proteins are indicated in kilodaltons to the left of both panels.
Article Snippet: When a sample of media conditioned by
Techniques: Western Blot, Transfection, SDS Page, Marker